In recent years experimental high-throughput methods, like microarrays, Yeast two hybrid, HMS and TAP have provided new insights into the complex processes within the cell. However, decreasing experimental costs lead to an exponentially increasing amount of data. To draw meaningful conclusions and to generate useful models from this information, computer supported analysis becomes necessary.

By employing microarry experiments we could demonstrate that the mouse tetradecanoyl phorbol acetate induced sequence 7 (TIS7) is a novel transcriptional co-repressor that can associate with the mammalian SIN3 complex[1]. The transcriptional repression of endogenous genes by tis7 overexpression is HDAC dependent. Thus, we propose TIS7 as a transcriptional co-repressor affecting the expression of specific genes in a HDAC activity-dependent manner during cell fate decisions, e.g. scattering.